What does chloroquine do in transfection

Discussion in 'Canada Pharmaceuticals Online' started by Spiny, 01-Mar-2020.

  1. Riooo Well-Known Member

    What does chloroquine do in transfection


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    Follow all directions on your prescription label. Do not take this medicine in larger or smaller amounts or for longer than recommended. To prevent malaria Start taking the medicine 2 weeks before entering an area where malaria is common. Chloroquine is usually taken once per week on the same day each week. So far we detected much higher transfection efficiency with Chloroquine than conventional CaPO4 transfection at 24 h. We use two different conditions with %2 FBS and %10 FBS. At the first day %10. Effects of chloroquine? Chloroquine is a relatively well-tolerated. medicine. The most common adverse reactions reported are stomach pain, nausea, vomiting, and headache. These side effects can often be lessened by taking chloroquine with food. Chloroquine may also cause itching in some people. All medicines may have some side effects.

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    What does chloroquine do in transfection

    Chloroquine MedlinePlus Drug Information, Questions with answers in CHLOROQUINE Science topic

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  3. The luciferase activity was also enhanced in the presence of primaquine, a chloroquine analogue, but was not increased when transfection was performed in the presence of ammonium chloride, methylamine, spermine, or monensin, compounds known to neutralize the pH of the endocytotic vesicle lumen as chloroquine does. Chloroquine enters cells and.

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    Transfection efficiencies will vary with cell type Can be used only for transient transfection Typically produces less than 10% delivery in primary cells Carefully transfer the transfection mix to the Lenti-X 293T packaging cells. Add the transfection mix dropwise being careful not to dislodge the cells. Incubate the cells for 18 h, or until the following morning. The following morning, carefully aspirate the media. Replace the media with 15 mL of DMEM complete. To understand how chloroquine CQ enhances transgene expression in polycation-based, nonviral gene delivery systems, a number of CQ analogues with variations in the aliphatic amino side chain or in the aromatic ring are synthesized and investigated.

     
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